Research Focus Area:
Treatment, Informatics, Detection, Diagnosis/ Prognosis
Type of Research:
Research Interest Statement:
In recent years, new immunotherapy drugs have revolutionized how clinicians treat patients with certain cancers like melanoma. For some patients, immunotherapy can effectively eliminate cancer from the body ? unfortunately in other patients these drugs show little benefit. Dr. Alan Tackett is interested in understanding why some patients show limited response to immunotherapies and then leveraging this information to design new cancer therapies to help all patients realize the lifesaving benefits of immunotherapy. His work is specifically focused on immunotherapy treatment of patients with metastatic melanoma. Towards these efforts, Dr. Tackett leads a translational team of researchers, oncologists, surgeons, and pathologists at the UAMS Winthrop P. Rockefeller Cancer Institute.
NIH/Nat. Inst. of General Medical Sciences – 1R24GM137786
“IDeA National Resource for Quantitative Proteomics”
08/20/20 – 05/31/25
NIH/Nat. Inst. of General Medical Sciences – 2P20GM103429
“Partnerships for Biomedical Research in Arkansas”
05/01/20 – 04/30/25
NIH/Nat. Cancer Institute – 1R01CA236209
“Identification of Druggable Targets to Complement Melanoma Therapy”
06/15/19 – 05/31/24
Kore RA, Henson JC, Hamzah RN, [et al., including Tackett AJ]. Author Correction: Molecular events in MSC exosome mediated cytoprotection in cardiomyocytes. Scientific reports. 2022 12(1):7874. PMID: 35551219. PMCID: PMC9098878.
Li D, Yu X, Kottur J, [et al., including Tackett AJ]. Discovery of a dual WDR5 and Ikaros PROTAC degrader as an anti-cancer therapeutic. Oncogene. 2022. PMID: 35525905.
Wardell CP, Darrigues E, De Loose A, [et al., including Tackett AJ]. Genomic and Transcriptomic Profiling of Brain Metastases. Cancers. 2021 13(22). PMID: 34830758. PMCID: PMC8615723.
Paidi SK, Troncoso JR, Raj P, [et al., including Tackett AJ]. Raman spectroscopy and machine learning reveals early tumor microenvironmental changes induced by immunotherapy. Cancer research. 2021. PMID: 34645610.
Delgado M, Washam CL, Urbaniak A, [et al., including Tackett AJ]. Phosphoproteomics Provides Novel Insights into the Response of Primary Acute Lymphoblastic Leukemia Cells to Microtubule Depolymerization in G1 Phase of the Cell Cycle. ACS omega. 2021 6(38):24949-24959. PMID: 34604676. PMCID: PMC8482483.
Xu C, Meng F, Park KS, [et al., including Tackett AJ]. A NSD3-targeted PROTAC suppresses NSD3 and cMyc oncogenic nodes in cancer cells. Cell chemical biology. 2021. PMID: 34469831.
Wang Y, Liu S, Yang Z, [et al., including Tackett AJ]. Anti-PD-1/L1 lead-in before MAPK inhibitor combination maximizes antitumor immunity and efficacy. Cancer cell. 2021. PMID: 34416167.
Ahn JH, Davis ES, Daugird TA, [et al., including Tackett AJ]. Phase separation drives aberrant chromatin looping and cancer development. Nature. 2021. PMID: 34163069.
Chappell K, Tackett A, Manna K, [et al.]. Multi-omics data integration reveals correlated regulatory features of triple negative breast cancer. Molecular omics. 2021. PMID: 34142686.
Reed MR, Maddukuri L, Ketkar A, [et al., including Tackett AJ]. Inhibition of tryptophan 2,3-dioxygenase impairs DNA damage tolerance and repair in glioma cells. NAR cancer. 2021 3(2):zcab014. PMID: 33870196. PMCID: PMC8034706.
Fan H, Guo Y, Tsai YH, [et al., including Tackett AJ]. A conserved BAH module within mammalian BAHD1 connects H3K27me3 to Polycomb gene silencing. Nucleic acids research. 2021 49(8):4441-4455. PMID: 33823544. PMCID: PMC8096256.
Xu C, Tsai YH, Galbo PM, [et al., including Tackett AJ]. Cistrome analysis of YY1 uncovers a regulatory axis of YY1:BRD2/4-PFKP during tumorigenesis of advanced prostate cancer. Nucleic acids research. 2021. PMID: 33849067.
Kelliher J, Tackett A, Ghosal G, Leung JWC. New answers to the old RIDDLE: RNF168 and the DNA damage response pathway. The FEBS journal. 2021. PMID: 33797206.
Jenkins SV, Alimohammadi M, Terry AS, [et al., including Tackett AJ]. Dysbiotic stress increases the sensitivity of the tumor vasculature to radiotherapy and c-Met inhibitors. Angiogenesis. 2021. PMID: 33629198.
Li J, Galbo PM Jr, Gong W, [et al., including Tackett AJ]. ZMYND11-MBTD1 induces leukemogenesis through hijacking NuA4/TIP60 acetyltransferase complex and a PWWP-mediated chromatin association mechanism. Nature communications. 2021 12(1):1045. PMID: 33594072.
Mohan M, Tackett A, Kumar M, [et al.]. Bone remineralization of lytic lesions in multiple myeloma - The Arkansas experience. Bone. 2021 146:115876. PMID: 33556629.
Kore RA, Wang X, Ding Z, [et al., including Tackett AJ]. MSC exosome-mediated cardioprotection in ischemic mouse heart comparative proteomics of infarct and peri-infarct areas. Molecular and cellular biochemistry. 2021. PMID: 33423165.
Graw S, Tackett A, Chappell K, [et al.]. Multi-omics data integration considerations and study design for biological systems and disease. Molecular omics. 2020. PMID: 33347526.
Hong A, Piva M, Liu S, [et al., including Tackett AJ]. Durable Suppression of Acquired MEK Inhibitor Resistance in Cancer by Sequestering MEK from ERK and Promoting Anti-Tumor T-cell Immunity. Cancer discovery. 2020. PMID: 33318037.
Ramirez AM, Beenken KE, Byrum SD, [et al., including Tackett AJ]. SarA plays a predominant role in controlling the production of extracellular proteases in the diverse clinical isolates of Staphylococcus aureus LAC and UAMS-1. Virulence. 2020 11(1):1738-1762. PMID: 33258416.
Koss B, Boerma M, Shields BD, [et al., including Tackett AJ]. Epigenetic Control of Cdkn2a.Arf Protects Tumor-Infiltrating Lymphocytes from Metabolic Exhaustion. Cancer research. 2020. PMID: 33004350.
Karaduta O, Glazko G, Dvanajscak Z, [et al., including Tackett A]. Resistant starch slows the progression of CKD in the 5/6 nephrectomy mouse model. Physiological reports. 2020 8(19):e14610. PMID: 33038060. PMCID: PMC7547583.
Mao XW, Nishiyama NC, Byrum SD, [et al., including Tackett AJ]. Spaceflight induces oxidative damage to blood-brain barrier integrity in a mouse model. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2020. PMID: 32981077.
Taylor EM, Byrum SD, Edmondson JL, [et al., including Tackett AJ]. Proteogenomic analysis of melanoma brain metastases from distinct anatomical sites identifies pathways of metastatic progression. Acta neuropathologica communications. 2020 8(1):157. PMID: 32891176. PMCID: PMC7487560.
Harrison DK, Tackett A, Waldrip ZJ, [et al.]. DNA-PKcs Inhibition Extends Allogeneic Skin Graft Survival. Transplantation. 2020. PMID: 32890138.
Storey AJ, Naceanceno KS, Lan RS, [et al., including Tackett AJ]. ProteoViz: a tool for the analysis and interactive visualization of phosphoproteomics data. Molecular omics. 2020. PMID: 32347222.
Davis LE, Shalin SC, Tackett AJ. Utility of histone H3K27me3 and H4K20me as diagnostic indicators of melanoma. Melanoma research. 2020 30(2):159-165. PMID: 32142497. PMCID: PMC7080300.
Storey AJ, Hardman RE, Byrum SD, [et al., including Tackett AJ]. Accurate and Sensitive Quantitation of the Dynamic Heat Shock Proteome Using Tandem Mass Tags. Journal of proteome research. 2020 19(3):1183-1195. PMID: 32027144. PMCID: PMC7241437.
Ramirez AM, Byrum SD, Beenken KE, [et al., including Tackett AJ]. Exploiting Correlations between Protein Abundance and the Functional Status of saeRS and sarA To Identify Virulence Factors of Potential Importance in the Pathogenesis of Staphylococcus aureus Osteomyelitis. ACS infectious diseases. 2020 6(2):237-249. PMID: 31722523. PMCID: PMC7294808.
Trentzsch M, Nyamugenda E, Miles TK, [et al., including Tackett AJ]. Delivery of phosphatidylethanolamine blunts stress in hepatoma cells exposed to elevated palmitate by targeting the endoplasmic reticulum. Cell death discovery. 2020 6:8. PMID: 32123584. PMCID: PMC7028721.
Taylor EM, Koss B, Davis LE, Tackett AJ. Histone Modifications as Biomarkers for Immunotherapy. Methods in molecular biology (Clifton, N.J.). 2020 2055:213-228. PMID: 31502154.
Waldrip ZJ, Jenjaroenpun P, DeYoung O, [et al., including Tackett AJ]. Genome-wide Cas9 binding specificity in Saccharomyces cerevisiae. PeerJ. 2020 8:e9442. PMID: 32821531. PMCID: PMC7395602.
Kore RA, Henson JC, Hamzah RN, [et al., including Tackett AJ]. Molecular events in MSC exosome mediated cytoprotection in cardiomyocytes. Scientific reports. 2019 9(1):19276. PMID: 31848380. PMCID: PMC6917778.
Ren Z, Ahn JH, Liu H, [et al., including Tackett AJ]. PHF19 promotes multiple myeloma tumorigenicity through PRC2 activation and broad H3K27me3 domain formation. Blood. 2019 134(14):1176-1189. PMID: 31383640. PMCID: PMC6776795.
Cragle CE, MacNicol MC, Byrum SD, [et al., including Tackett AJ]. Musashi interaction with poly(A)-binding protein is required for activation of target mRNA translation. The Journal of biological chemistry. 2019 294(28):10969-10986. PMID: 31152063. PMCID: PMC6635449.
Chiang TC, Koss B, Su L, [et al., including Tackett AJ]. Effect of Sulforaphane and 5-Aza-2'-Deoxycytidine on Melanoma Cell Growth. Medicines (Basel, Switzerland). 2019 6(3). PMID: 31252639. PMCID: PMC6789461.
Mao XW, Nishiyama NC, Byrum SD, [et al., including Tackett AJ]. Characterization of mouse ocular response to a 35-day spaceflight mission: Evidence of blood-retinal barrier disruption and ocular adaptations. Scientific reports. 2019 9(1):8215. PMID: 31160660. PMCID: PMC6547757.
Lo S, Byrum SD, Tackett AJ, Parajuli N. Cold Storage Increases Albumin and Advanced Glycation-End Product-Albumin Levels in Kidney Transplants: A Possible Cause for Exacerbated Renal Damage. Transplantation direct. 2019 5(6):e454. PMID: 31723591. PMCID: PMC6791592.
Garcia-Rill E, Tackett AJ, Byrum SD, [et al.]. Local and Relayed Effects of Deep Brain Stimulation of the Pedunculopontine Nucleus. Brain sciences. 2019 9(3). PMID: 30889866. PMCID: PMC6468768.
Shields BD, Koss B, Taylor EM, [et al., including Tackett AJ]. Loss of E-Cadherin Inhibits CD103 Antitumor Activity and Reduces Checkpoint Blockade Responsiveness in Melanoma. Cancer research. 2019 79(6):1113-1123. PMID: 30674537. PMCID: PMC6420873.
West KL, Byrum SD, Mackintosh SG, [et al., including Tackett AJ]. Proteomic characterization of the arsenic response locus in S. cerevisiae. Epigenetics. 2019 14(2):130-145. PMID: 30739529. PMCID: PMC6557609.
Uddin MB, Roy KR, Hosain SB, [et al., including Tackett AJ]. An N6-methyladenosine at the transited codon 273 of p53 pre-mRNA promotes the expression of R273H mutant protein and drug resistance of cancer cells. Biochemical pharmacology. 2019 160:134-145. PMID: 30578766. PMCID: PMC6407121.
Davis LE, Shalin SC, Tackett AJ. Current state of melanoma diagnosis and treatment. Cancer biology & therapy. 2019 20(11):1366-1379. PMID: 31366280. PMCID: PMC6804807.
Byrum SD, Tackett A, Washam CL, [et al.]. Continuous Developmental and Early Life Trichloroethylene Exposure Promoted DNA Methylation Alterations in Polycomb Protein Binding Sites in Effector/Memory CD4+ T Cells. Frontiers in immunology. 2019 10:2016. PMID: 31555266. PMCID: PMC6724578.
Zybailov BL, Glazko GV, Rahmatallah Y, [et al., including Tackett AJ]. Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats. PloS one. 2019 14(1):e0199274. PMID: 30699108. PMCID: PMC6353070.
Cheema AK, Byrum SD, Sharma NK, [et al., including Tackett AJ]. Proteomic Changes in Mouse Spleen after Radiation-Induced Injury and its Modulation by Gamma-Tocotrienol. Radiation research. 2018 190(5):449-463. PMID: 30070965. PMCID: PMC6297072.
Storey AJ, Wang HP, Protacio RU, [et al., including Tackett AJ]. Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot. Epigenetics & chromatin. 2018 11(1):64. PMID: 30373637. PMCID: PMC6205778.
Byrum SD, Loughran AJ, Beenken KE, [et al., including Tackett AJ]. Label-Free Proteomic Approach to Characterize Protease-Dependent and -Independent Effects of sarA Inactivation on the Staphylococcus aureus Exoproteome. Journal of proteome research. 2018 17(10):3384-3395. PMID: 30209945. PMCID: PMC6209314.
Kriss CL, Gregory-Lott E, Storey AJ, [et al., including Tackett AJ]. In Vivo Metabolic Tracing Demonstrates the Site-Specific Contribution of Hepatic Ethanol Metabolism to Histone Acetylation. Alcoholism, clinical and experimental research. 2018 42(10):1909-1923. PMID: 30030934. PMCID: PMC6208134.
Firwana B, Avaritt N, Shields B, [et al., including Tackett AJ]. Do checkpoint inhibitors rely on gut microbiota to fight cancer?. Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners. 2018 24(6):468-472. PMID: 28625074.
Mao XW, Byrum S, Nishiyama NC, [et al., including Tackett AJ]. Impact of Spaceflight and Artificial Gravity on the Mouse Retina: Biochemical and Proteomic Analysis. International journal of molecular sciences. 2018 19(9). PMID: 30154332. PMCID: PMC6165321.
Zhang X, Zhang S, Liu X, [et al., including Tackett AJ]. Oxidation resistance 1 is a novel senolytic target. Aging cell. 2018 17(4):e12780. PMID: 29766639. PMCID: PMC6052462.
Vlachos A, Tackett A, Osorio DS, [et al.]. Increased Prevalence of Congenital Heart Disease in Children With Diamond Blackfan Anemia Suggests Unrecognized Diamond Blackfan Anemia as a Cause of Congenital Heart Disease in the General Population: A Report of the Diamond Blackfan Anemia Registry. Circulation. Genomic and precision medicine. 2018 11(5):e002044. PMID: 29748317. PMCID: PMC5951415.
McSweeney JC, Hudson TJ, Prince L, [et al., including Tackett AJ]. Impact of the INBRE summer student mentored research program on undergraduate students in Arkansas. Advances in physiology education. 2018 42(1):123-129. PMID: 29446316. PMCID: PMC6334933.
Arthur JM, Tackett A, Karakala N, Edmondson RD. Proteomic Analysis for Identification of Biomarkers that Predict Severe Acute Kidney Injury. Nephron. 2018 140(2):129-133. PMID: 29990988. PMCID: PMC6398162.
Bolouri H, Farrar JE, Triche T Jr, [et al., including Tackett A]. The molecular landscape of pediatric acute myeloid leukemia reveals recurrent structural alterations and age-specific mutational interactions. Nature medicine. 2018 24(1):103-112. PMID: 29227476. PMCID: PMC5907936.
Rahmatallah Y, Tackett A, Khaidakov M, [et al.]. Platform-independent gene expression signature differentiates sessile serrated adenomas/polyps and hyperplastic polyps of the colon. BMC medical genomics. 2017 10(1):81. PMID: 29284484. PMCID: PMC5745747.
Wang P, Byrum S, Fowler FC, [et al., including Tackett AJ]. Proteomic identification of histone post-translational modifications and proteins enriched at a DNA double-strand break. Nucleic acids research. 2017 45(19):10923-10940. PMID: 29036368. PMCID: PMC5737490.
Oliveira C, Gunderman L, Coles CA, [et al., including Tackett AJ]. 16S rRNA Gene-Based Metagenomic Analysis of Ozark Cave Bacteria. Diversity. 2017 9(3). PMID: 29551950. PMCID: PMC5856467.
Miousse IR, Chang J, Shao L, [et al., including Tackett AJ]. Inter-Strain Differences in LINE-1 DNA Methylation in the Mouse Hematopoietic System in Response to Exposure to Ionizing Radiation. International journal of molecular sciences. 2017 18(7). PMID: 28677663. PMCID: PMC5535921.
Mahmoud F, Shields B, Makhoul I, [et al., including Tackett AJ]. Immune surveillance in melanoma: From immune attack to melanoma escape and even counterattack. Cancer biology & therapy. 2017 18(7):451-469. PMID: 28513269. PMCID: PMC5639850.
Shields BD, Mahmoud F, Taylor EM, [et al., including Tackett AJ]. Indicators of responsiveness to immune checkpoint inhibitors. Scientific reports. 2017 7(1):807. PMID: 28400597. PMCID: PMC5429745.
Hartman JH, Miller GP, Caro AA, [et al., including Tackett AJ]. 1,3-Butadiene-induced mitochondrial dysfunction is correlated with mitochondrial CYP2E1 activity in Collaborative Cross mice. Toxicology. 2017 378:114-124. PMID: 28082109. PMCID: PMC5319481.
Holthoff ER, Byrum SD, Mackintosh SG, [et al., including Tackett AJ]. Vulvar squamous cell carcinoma aggressiveness is associated with differential expression of collagen and STAT1. Clinical proteomics. 2017 14:40. PMID: 29225558. PMCID: PMC5717999.
Byrum SD, Burdine MS, Orr L, [et al., including Tackett AJ]. Time- and radiation-dose dependent changes in the plasma proteome after total body irradiation of non-human primates: Implications for biomarker selection. PloS one. 2017 12(3):e0174771. PMID: 28350824. PMCID: PMC5370149.
Kim Wiese A, Schluterman Burdine M, Turnage RH, [et al., including Tackett AJ]. DNA-PKcs controls calcineurin mediated IL-2 production in T lymphocytes. PloS one. 2017 12(7):e0181608. PMID: 28750002. PMCID: PMC5531461.
Miousse IR, Pathak R, Garg S, [et al., including Tackett AJ]. Short-term dietary methionine supplementation affects one-carbon metabolism and DNA methylation in the mouse gut and leads to altered microbiome profiles, barrier function, gene expression and histomorphology. Genes & nutrition. 2017 12:22. PMID: 28904640. PMCID: PMC5588631.
Ayyadevara S, Balasubramaniam M, Suri P, [et al., including Tackett AJ]. Proteins that accumulate with age in human skeletal-muscle aggregates contribute to declines in muscle mass and function in Caenorhabditis elegans. Aging. 2016 8(12):3486-3497. PMID: 27992858. PMCID: PMC5270681.
Blair LP, Liu Z, Labitigan RL, [et al., including Tackett AJ]. KDM5 lysine demethylases are involved in maintenance of 3'UTR length. Science advances. 2016 2(11):e1501662. PMID: 28138513. PMCID: PMC5262454.
Boerma M, Sridharan V, Mao XW, [et al., including Tackett AJ]. Effects of ionizing radiation on the heart. Mutation research. 2016 770(Pt B):319-327. PMID: 27919338. PMCID: PMC5144922.
Prior S, Miousse IR, Nzabarushimana E, [et al., including Tackett AJ]. Densely ionizing radiation affects DNA methylation of selective LINE-1 elements. Environmental research. 2016 150:470-481. PMID: 27419368. PMCID: PMC5003736.
Ayyadevara S, Balasubramaniam M, Parcon PA, [et al., including Tackett AJ]. Proteins that mediate protein aggregation and cytotoxicity distinguish Alzheimer's hippocampus from normal controls. Aging cell. 2016 15(5):924-39. PMID: 27448508. PMCID: PMC5013017.
Loughran AJ, Gaddy D, Beenken KE, [et al., including Tackett AJ]. Impact of sarA and Phenol-Soluble Modulins on the Pathogenesis of Osteomyelitis in Diverse Clinical Isolates of Staphylococcus aureus. Infection and immunity. 2016 84(9):2586-94. PMID: 27354444. PMCID: PMC4995912.
Shields BD, Tackett AJ, Shalin SC. Proteomics and melanoma: a current perspective. Global dermatology. 2016 3(4):366-370. PMID: 30214824. PMCID: PMC6133306.
Yale K, Tackett AJ, Neuman M, [et al.]. Phosphorylation-Dependent Targeting of Tetrahymena HP1 to Condensed Chromatin. mSphere. 2016 1(4). PMID: 27579368. PMCID: PMC4999919.
Mahmoud F, Shields B, Makhoul I, [et al., including Tackett AJ]. Role of EZH2 histone methyltrasferase in melanoma progression and metastasis. Cancer biology & therapy. 2016 17(6):579-91. PMID: 27105109. PMCID: PMC4990393.
Atanassov BS, Mohan RD, Lan X, [et al., including Tackett AJ]. ATXN7L3 and ENY2 Coordinate Activity of Multiple H2B Deubiquitinases Important for Cellular Proliferation and Tumor Growth. Molecular cell. 2016 62(4):558-71. PMID: 27132940. PMCID: PMC4874879.
Ayyadevara S, Mercanti F, Wang X, [et al., including Tackett AJ]. Age- and Hypertension-Associated Protein Aggregates in Mouse Heart Have Similar Proteomic Profiles. Hypertension (Dallas, Tex. : 1979). 2016 67(5):1006-13. PMID: 26975704. PMCID: PMC4833546.
Wu TP, Wang T, Seetin MG, [et al., including Tackett A]. DNA methylation on N(6)-adenine in mammalian embryonic stem cells. Nature. 2016 532(7599):329-33. PMID: 27027282. PMCID: PMC4977844.
Heard ME, Besio R, Weis M, [et al., including Tackett AJ]. Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation. PLoS genetics. 2016 12(4):e1006002. PMID: 27119146. PMCID: PMC4847768.
Sengupta D, Tackett AJ. Proteomic Findings in Melanoma. Journal of proteomics & bioinformatics. 2016 9(4). PMID: 27274624. PMCID: PMC4888906.
Salinas E, Byrum SD, Moreland LE, [et al., including Tackett AJ]. Identification of Viral and Host Proteins That Interact with Murine Gammaherpesvirus 68 Latency-Associated Nuclear Antigen during Lytic Replication: a Role for Hsc70 in Viral Replication. Journal of virology. 2016 90(3):1397-413. PMID: 26581985. PMCID: PMC4719591.
Shields B, Shalin SC, Tackett AJ. Microscopes and Mass Spectrometers. Journal of proteomics & bioinformatics. 2016 9(Suppl 10). PMID: 29910559. PMCID: PMC5998814.
Byrum SD, Burdine MS, Orr L, [et al., including Tackett AJ]. A Quantitative Proteomic Analysis of Urine from Gamma-Irradiated Non-Human Primates. Journal of proteomics & bioinformatics. 2016 9(Suppl 10). PMID: 26962295. PMCID: PMC4780756.